Native promoters of Corynebacterium glutamicum and its application in l-lysine production

@article{Shang2017NativePO,
  title={Native promoters of Corynebacterium glutamicum and its application in l-lysine production},
  author={Xiuling Shang and Xin Chai and Xuemei Lu and Yuan Li and Yun Zhang and Guoqiang Wang and Chen Zhang and Shuwen Liu and Yu Zhang and Jiyin Ma and Tingyi Wen},
  journal={Biotechnology Letters},
  year={2017},
  volume={40},
  pages={383-391}
}
To identify useful native promoters of Corynebacterium glutamicum for fine-tuning of gene expression in metabolic engineering. Sixteen native promoters of C. glutamicum were characterized. These promoters covered a strength range of 31-fold with small increments and exhibited relatively stable activity during the whole growth phase using β-galactosidase as the reporter. The mRNA level and enzymatic activity of the lacZ reporter gene exhibited high correlation (R 2 = 0.96) under the control of… CONTINUE READING