Native polyacrylamide gels.

Abstract

Usually proteins are separated by polyacrylamide gel electrophoresis (PAGE) in the presence of a detergent and under (heat-) denaturing and (non- or) reducing conditions. The most commonly used detergent is sodium dodecyl sulfate (SDS). The major function of SDS is to shield the respective charge of the proteins present in the mixture to be analyzed and to… (More)
DOI: 10.1007/978-1-61779-821-4_5

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Cite this paper

@article{Arndt2012NativePG, title={Native polyacrylamide gels.}, author={Claudia Arndt and Stefanie Koristka and Holger Bartsch and Michael Bachmann}, journal={Methods in molecular biology}, year={2012}, volume={869}, pages={49-53} }