Native chromatin immunoprecipitation.

@article{Thorne2004NativeCI,
  title={Native chromatin immunoprecipitation.},
  author={Alan W. Thorne and Fiona A. Myers and Tim R Hebbes},
  journal={Methods in molecular biology},
  year={2004},
  volume={287},
  pages={21-44}
}
Chromatin immunoprecipitation (ChIP) is a technique widely used for determining the genomic location of modified histones and other chromatin-associated factors. Here we describe the methodology we have used in our laboratory for the immunoprecipitation of chromatin isolated from cells in the absence of crosslinking. Chromatin released from nuclei by micrococcal nuclease digestion is centrifuged through sucrose gradients to allow selection of mono- or dinucleosomes. This allows a protein or… CONTINUE READING

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