Native chemical ligation at glutamine.


The desulfurization reaction introduced by Yan and Dawson as a postnative chemical ligation step greatly expanded the scope of ligation chemistry beyond Xaa-Cys (Xaa is any amino acid) by making ligation at Xaa-Phe, Xaa-Val, Xaa-Lys, Xaa-Leu, Xaa-Thr, and Xaa-Pro junctions accessible in the synthesis of functional proteins. A new ligation site based on Xaa-Gln utilizing γ-mercaptoglutamine is reported, and several examples on the efficiency of ligation coupled with desulfurization are provided.

DOI: 10.1021/ol300254y
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@article{Siman2012NativeCL, title={Native chemical ligation at glutamine.}, author={Peter Siman and Subramanian Vedhanarayanan Karthikeyan and Ashraf Brik}, journal={Organic letters}, year={2012}, volume={14 6}, pages={1520-3} }