Native RNA purification by gel filtration chromatography.

In vitro transcription of RNA from DNA templates by T7 RNA polymerase allows for the generation of large quantities of RNA suitable for many downstream applications. The resulting RNA can be purified by a number of methodologies. Herein, we describe the native isolation of RNA molecules by FPLC purification using Superdex 75 or 200 gel filtration columns… CONTINUE READING