National standardization of ZAP-70 determination by flow cytometry: the French experience.

@article{GarffTavernier2007NationalSO,
  title={National standardization of ZAP-70 determination by flow cytometry: the French experience.},
  author={Magali Le Garff-Tavernier and Michel Ticchioni and Martine Brissard and C{\'e}line Salmon and Sophie Raynaud and Frederic Davi and Alain Bernard and H{\'e}l{\`e}ne Merle-B{\'e}ral and Florence Ajchenbaum-Cymbalista and R{\'e}mi Letestu},
  journal={Cytometry. Part B, Clinical cytometry},
  year={2007},
  volume={72 2},
  pages={103-8}
}
BACKGROUND ZAP-70, after being considered as a potential surrogate for VH mutational status, has seen its own prognostic value emerge. We aimed at standardizing a simple, fast, and reproducible flow cytometry method. METHODS AntiZAP-70 antibody 2F3.2 was used with indirect labeling and secondary anti-IgG2a antibody. The reference values for the expression of the results were determined on 45 normal blood samples. ZAP-70 protein expression was investigated in 192 CLL samples. The indirect… CONTINUE READING