Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters.

@article{Core2008NascentRS,
  title={Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters.},
  author={Leighton J. Core and Joshua J Waterfall and John T Lis},
  journal={Science},
  year={2008},
  volume={322 5909},
  pages={1845-8}
}
RNA polymerases are highly regulated molecular machines. We present a method (global run-on sequencing, GRO-seq) that maps the position, amount, and orientation of transcriptionally engaged RNA polymerases genome-wide. In this method, nuclear run-on RNA molecules are subjected to large-scale parallel sequencing and mapped to the genome. We show that peaks of promoter-proximal polymerase reside on approximately 30% of human genes, transcription extends beyond pre-messenger RNA 3' cleavage, and… CONTINUE READING
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  • M. Kane, Biochim Biophys Acta
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