Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters.

Abstract

RNA polymerases are highly regulated molecular machines. We present a method (global run-on sequencing, GRO-seq) that maps the position, amount, and orientation of transcriptionally engaged RNA polymerases genome-wide. In this method, nuclear run-on RNA molecules are subjected to large-scale parallel sequencing and mapped to the genome. We show that peaks… (More)
DOI: 10.1126/science.1162228

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@article{Core2008NascentRS, title={Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters.}, author={Leighton J. Core and Joshua J. Waterfall and John T Lis}, journal={Science}, year={2008}, volume={322 5909}, pages={1845-8} }