Narrowing of T-cell receptor beta variable repertoire during symptomatic herpesvirus infection in transplant patients.

Abstract

Primary infection or recrudescence of latent virus infection in transplant recipients can be manifested either as asymptomatic or symptomatic disease. Here we show that symptomatic human cytomegalovirus (HCMV) or Epstein-Barr virus (EBV) infection or recrudescence following solid organ transplantation (SOT) was coincident with a dramatic skewing of T-cell receptor beta variable (TRBV) repertoire, with expansions of monoclonal/oligoclonal clonotypes. As the clinical symptoms resolved, the peripheral blood repertoire reverted to a more diverse distribution. In contrast, SOT recipients with asymptomatic or no viral infection or recrudescence showed minimal or no skewing of the T-cell receptor repertoire to maintain peripheral blood repertoire diversity. More importantly, we show that large monoclonal/oligoclonal repertoire expansions are associated with the loss of HCMV-specific T-cell function observed in SOT patients undergoing symptomatic viral infection or recrudescence, whereas SOT recipients who maintain peripheral blood TRBV repertoire diversity and functional antigen-specific T-cell responses can resist clinical symptomatic disease in spite of high levels of viral load.

DOI: 10.1038/icb.2009.74

Cite this paper

@article{Wynn2010NarrowingOT, title={Narrowing of T-cell receptor beta variable repertoire during symptomatic herpesvirus infection in transplant patients.}, author={Katherine K. Wynn and Tania Crough and Scott Bryan Campbell and Keith D. McNeil and Andrew John Galbraith and Denis J. Moss and Sharon L. Silins and Scott C Bell and Rajiv Khanna}, journal={Immunology and cell biology}, year={2010}, volume={88 2}, pages={125-35} }