Nanoscale separations combined with tandem mass spectrometry.


High-efficiency separations of peptide mixtures, tryptic digest and other biological compounds have been achieved using nanoscale packed capillaries and capillary zone electrophoresis (CZE). The coaxial continuous-flow fast atom bombardment design is an excellent interface for coupling these separation techniques with mass spectrometry (MS). In addition, this interface is very useful for the acquisition of MS-MS data from compounds separated by nanoscale packed capillary liquid chromatography and CZE. Structurally informative daughter-ion spectra can be obtained at the low picomole to femtomole level.

Cite this paper

@article{Deterding1991NanoscaleSC, title={Nanoscale separations combined with tandem mass spectrometry.}, author={Leesa J. Deterding and M. Arthur Moseley and Kenneth B. Tomer and James W . Jorgenson}, journal={Journal of chromatography}, year={1991}, volume={554 1-2}, pages={73-82} }