Nanoscale mapping and functional analysis of individual adhesins on living bacteria

@article{Duprs2005NanoscaleMA,
  title={Nanoscale mapping and functional analysis of individual adhesins on living bacteria},
  author={Vincent Dupr{\`e}s and Franco Menozzi and Camille Locht and Brian H. Clare and Nicholas L Abbott and St{\'e}phane Cuenot and Coralie Bompard and Dominique Raze and Yves F Dufr{\^e}ne},
  journal={Nature Methods},
  year={2005},
  volume={2},
  pages={515-520}
}
Although much progress has been made in the identification and characterization of adhesins borne by pathogenic bacteria, the molecular details underlying their interaction with host receptors remain largely unknown owing to the lack of appropriate probing techniques. Here we report a method, based on atomic force microscopy (AFM) with tips bearing biologically active molecules, for measuring the specific binding forces of individual adhesins and for mapping their distribution on the surface of… CONTINUE READING

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