Nanoscale elucidation of Na,K-ATPase isoforms in dendritic spines

  title={Nanoscale elucidation of Na,K-ATPase isoforms in dendritic spines},
  author={Thomas Liebmann and Hans Blom and Anita Aperia and Hjalmar Brismar},
  journal={Optical Nanoscopy},
BackgroundThe dimensions of neuronal synapses suggest that optical super-resolution imaging methods are necessary for thorough investigation of protein distributions and interactions. Nanoscopic evaluation of neuronal samples has presented practical hurdles, but advancing methods are making synaptic protein topology and quantification measurements feasible. This work explores the application of Photoactivated Localization Microscopy (PALM) pointillistic super-resolution imaging for… 

Sodium pump organization in dendritic spines

This work has investigated the principal energy transformer in the brain, i.e., the Na+,K+-ATPase (or sodium pump), an essential protein responsible for maintaining resting membrane potential and a major controller of intracellular ion homeostasis.

Regulation of Neuronal Na,K-ATPase by Extracellular Scaffolding Proteins

It is reported that in rat primary hippocampal neurons, the clearance of Na+ ions is more efficient if Na,K-ATPase is laterally mobile in the membrane than if it is clustered and clustering, caused by cross-linking or by exposure to MONaKA, reduces the efficiency in restoring intracellular Na+.

Nanoscopic spine localization of Norbin, an mGluR5 accessory protein

The spatial relationship between Norbin, postsynaptic density protein 95 (PSD-95), actin and mGluR5 in spines is analyzed using super-resolution microscopy to provide more accurate information about sub-cellular protein localization than previously was possible.

Implications of localization and transport regulation of postsynaptic membrane proteins for synaptic function and psychiatric disorders

Regulation of postsynaptic membrane protein trafficking is described that adds to the fundamental knowledge of neuronal function and introduces new avenues for signal regulation.

Expression of Na/K-ATPase subunits in the human cochlea: a confocal and super-resolution microscopy study with special reference to auditory nerve excitation and cochlear implantation

The human auditory nerve displays distinct morphologic features represented in its molecular expression and it was found that electric signals generated via hair cells may not go uninterrupted across the spiral ganglion, but are locally processed.

An interaction between PRRT2 and Na+/K+ ATPase contributes to the control of neuronal excitability

Results demonstrate that PRRT2 is a physiological modulator of NKA function and suggest that an impaired NKA activity contributes to the hyperexcitability phenotype caused byPRRT2 deficiency.

Nanoscopy—imaging life at the nanoscale: a Nobel Prize achievement with a bright future

A grand scientific prize was awarded last year to three pioneering scientists, for their discovery and development of molecular ‘ON–OFF’ switching which, when combined with optical imaging, can be

The Role of αSAP97 and βSAP97 at the Glutamatergic Synapse

The scaffolding of synapse-associated protein-97 isoforms scaffold synaptic and extrasynaptic receptors and their role in receptor recycling and recycling are illustrated.



Spatial distribution of Na+-K+-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy

The found compartmentalized distribution provides a strong evidence for the confinement of neuronal Na+,K+-ATPase (α3 isoform) in the postsynaptic region of the spine and may have implications for the generation of local sodium gradients within the backbone and for the structural and functional interaction between the sodium pump and other synaptic proteins.

Nearest neighbor analysis of dopamine D1 receptors and Na+‐K+‐ATPases in dendritic spines dissected by STED microscopy

The STED dissected nanoscale topologies provide evidence for both a joint as well as a separated confinement of the D1 receptor and the Na+,K+‐ATPase in the postsynaptic areas of dendritic spines.

Super-Resolution Imaging Reveals That AMPA Receptors Inside Synapses Are Dynamically Organized in Nanodomains Regulated by PSD95

The observation that AMPARs are highly concentrated in nanodomains, instead of diffusively distributed in the PSD as generally thought, has important consequences on the understanding of excitatory neurotransmission.

Enlightening G-protein-coupled receptors on the plasma membrane using super-resolution photoactivated localization microscopy.

The application of super-resolution microscopy to study the spatial and temporal organization of GPCRs has brought new insights into receptor arrangement on the plasma membrane and the use of this powerful microscopy technique as a quantitative tool opens up the possibility for investigating and quantifying the number of molecules in biological assemblies and determining the protein stoichiometry in signalling complexes.

Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging

GCaMP5 fluorescence provides a more reliable measure of neuronal activity than its predecessor GCaMP3, which allows more sensitive detection of neural activity in vivo and may find widespread applications for cellular imaging in general.

STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis

It is shown that synaptotagmin I, a protein resident in the vesicle membrane, remains clustered in isolated patches on the presynaptic membrane regardless of whether the nerve terminals are mildly active or intensely stimulated, suggesting that at least some vesicles constituents remain together during recycling.

Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes

This work identified photoswitchable membrane probes and obtained super-resolution fluorescence images of cellular membranes, and demonstrated the photoswitching capabilities of eight commonly used membrane probes, each specific to the plasma membrane, mitochondria, the endoplasmic recticulum (ER) or lysosomes.

Molecular anatomy of the postsynaptic density

  • S. Okabe
  • Biology
    Molecular and Cellular Neuroscience
  • 2007