Nanopore direct RNA sequencing detects differential expression between human cell populations

  title={Nanopore direct RNA sequencing detects differential expression between human cell populations},
  author={Josie Gleeson and Tracy A. Lane and Paul J. Harrison and Wilfried Haerty and Michael B. Clark},
Accurately quantifying gene and isoform expression changes is essential to understanding cell functions, differentiation and disease. Therefore, a crucial requirement of RNA sequencing is identifying differential expression. The recent development of long-read direct RNA (dRNA) sequencing has the potential to overcome many limitations of short and long-read sequencing methods that require RNA fragmentation, cDNA synthesis or PCR. dRNA sequences native RNA and can encompass an entire RNA in a… 

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  • M. Hölzer
  • Environmental Science
    Molecular ecology resources
  • 2020
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