Nanoparticle-based bio-barcode assay for the detection of bluetongue virus.

  title={Nanoparticle-based bio-barcode assay for the detection of bluetongue virus.},
  author={Hui-qiong Yin and Min-xian Jia and Li-jun Shi and Shu Yang and Li-Ying Zhang and Qi-mo Zhang and Shen-qi Wang and Gang Li and Jingang Zhang},
  journal={Journal of virological methods},
  volume={178 1-2},
The ultrasensitive bio-barcode amplification assay (BCA) technique was developed for the specific detection of the outer-core protein VP7 of bluetongue virus (BTV). The target antigen VP7 was first captured by gold nanoparticles (GNPs) coated with polyclonal antibodies. Magnetic microparticles (MMP) coated with VP7 monoclonal antibody were then added to form a sandwich immuno-complex. After the immuno-complex was formed, signal DNA annealed to DNA strands covalently bound to the GNPs were… CONTINUE READING


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Molec - ular cloning and sequence analysis of the S 7 - ORF of bluetongu virus 5

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