Nanomolar affinity protein trans-splicing monitored in real-time by fluorophore-quencher pairs.

@article{Braner2017NanomolarAP,
  title={Nanomolar affinity protein trans-splicing monitored in real-time by fluorophore-quencher pairs.},
  author={Markus Braner and Ralph Wieneke and Robert Tamp{\'e}},
  journal={Chemical communications},
  year={2017},
  volume={53 3},
  pages={545-548}
}
High background originating from non-reacted, 'always-on' fluorescent probes remains a key unsolved problem in life science since washing procedures are not easily applicable. Covalent labeling approaches with simultaneous activation of fluorescence are advantageous to increase sensitivity and to reduce background signal. Here, we combined high-affinity… CONTINUE READING