Nanoliter scale PCR with TaqMan detection.

@article{Kalinina1997NanoliterSP,
  title={Nanoliter scale PCR with TaqMan detection.},
  author={Olga Kalinina and Irina Lebedeva and James O. Brown and Jack Silver},
  journal={Nucleic acids research},
  year={1997},
  volume={25 10},
  pages={
          1999-2004
        }
}
We monitored PCR in volumes of the order of 10 nl in glass microcapillaries using a fluorescence energy transfer assay in which fluorescence increases if product is made due to template-dependent nucleolytic degradation of an internally quenched probe (TaqMan assay). This assay detected single starting template molecules in dilutions of genomic DNA. The results suggest that it may be feasible to determine the number of template molecules in a sample by counting the number of positive PCRs in a… CONTINUE READING
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