Nanog A New Recruit to the Embryonic Stem Cell Orchestra

  title={Nanog A New Recruit to the Embryonic Stem Cell Orchestra},
  author={F. Cavaleri and H. Sch{\"o}ler},
In this issue of Cell, and add a new transcriptional operating system to the known Oct4 and Stat3 systems required for early embryonal stem cell potency and self-renewal. Nanog, a homeobox transcription factor, plays a crucial role in the second embryonic cell fate specification following formation of the blastocyst. 
[Two vital transcriptional factors Oct-4 and Nanog to keep the pluripotency and self-renewal of stem cells and related regulation network].
Oct-4 and Nanog are two critical transcriptional factors to keep pluripotency and self-renewal of stem cells in vivo and in vitro, and they usually express only in pluripotent cells and not inExpand
Chipping away at 'stemness'
Global gene-expression analyses of human embryonic stem cells confirm the involvement of some known genes in stem-cell function and identify some new candidate regulators of stem-cell growth. SupportExpand
Activated 5′flanking region of NANOGP8 in a self‐renewal environment is associated with increased sphere formation and tumor growth of prostate cancer cells
The role of NANOGP8 in cell proliferation has not been completely understood because of the lack of investigation into the regulation of its expression. Expand
Expression and Function of Pluripotency Genes in Adult Stem Cells
This chapter discusses whether adult somatic stem cells express the Oct4 and Nanog genes that are known to play a role in the self-renewal and pluripotency characteristics of ESC, and whether such expression plays arole in the potency of adult tissue derived stem cells. Expand
Molecular mechanisms on embryonic stem cell pluripotency
Derived from early embryo, embryonic stem (ES) cells have potential to self renew indefinitely and develop to any type of cells in human body (pluripotency). Several extrinsic signals such as LIF,Expand
SOX2 gene – master regulator of numerous cellular processes
The effects ofSOX2 overexpression is studied and some of the recent findings that have highlighted the important roles of SOX2 in the maintenance of pluripotency, proliferation, neural differentiation and in the regulation of the migration capacity of cells are presented. Expand
The Stem Cell Pluripotency Factor NANOG Activates Transcription with Two Unusually Potent Subdomains at Its C Terminus*
  • G. Pan, D. Pei
  • Medicine, Biology
  • Journal of Biological Chemistry
  • 2005
The evidence that NANOG behaves as a transcription activator with two unusually strong activation domains embedded in its C terminus is provided, suggesting that either WR or CD2 is sufficient for NANog to function as a transactivator. Expand
NaNog: A pluripotency homeobox (master) molecule
NaNog, the subject of this article, is a molecule implicated in regulating pluripotency that is required during organogenesis in foetal life and importantly during tissue repair in health and disease. Expand
DjPum, a homologue of Drosophila Pumilio, is essential to planarian stem cell maintenance
Through RNA interference (RNAi), it is demonstrated that gene silencing of DjPum dramatically reduces the number of neoblasts, thus supporting the intriguing hypothesis that stem cell maintenance may be an ancestral function of PUF proteins. Expand
Identification of two distinct transactivation domains in the pluripotency sustaining factor nanog
It is demonstrated that both regions N- and C- terminal to the homeodomain have transcription activities, and this unique arrangement of dual transactivators may confer nanog the flexibility and specificity to regulate downstream genes critical for both pluripotency and differentiation of stem cells. Expand


Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm. Expand
Multipotent cell lineages in early mouse development depend on SOX2 function.
The data suggest that maternal components could be involved in establishing early cell fate decisions and that a combinatorial code, requiring SOX2 and OCT4, specifies the first three lineages present at implantation. Expand
Requirement for Foxd3 in maintaining pluripotent cells of the early mouse embryo.
It is reported that Foxd3, a member of the forkhead family of transcriptional regulators, is required for maintenance of embryonic cells of the early mouse embryo and thatFoxd3-/- embryos can be rescued by a small number of wild-type cells. Expand
Functional Expression Cloning of Nanog, a Pluripotency Sustaining Factor in Embryonic Stem Cells
These findings establish a central role for Nanog in the transcription factor hierarchy that defines ES cell identity and confirm that Cytokine dependence, multilineage differentiation, and embryo colonization capacity are fully restored upon transgene excision. Expand
The Homeoprotein Nanog Is Required for Maintenance of Pluripotency in Mouse Epiblast and ES Cells
Nanog is a critical factor underlying pluripotency in both ICM and ES cells, and it is found that one of them, encoding the homeoprotein Nanog, was capable of maintaining ES cell self-renewal independently of LIF/Stat3. Expand
STAT3 activation is sufficient to maintain an undifferentiated state of mouse embryonic stem cells
It is strongly suggested that STAT3 activation is required and sufficient to maintain the undifferentiated state of ES cells. Expand
Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3.
Investigation of the role of STAT3 and inducible STAT3F in ES cells growing in the presence of LIF specifically abrogated self-renewal and promoted differentiation establish that STAT3 plays a central role in the maintenance of the pluripotential stem cell phenotype. Expand
Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells
It is demonstrated here that purified, recombinant LIF can substitute for DIA in the maintenance of totipotent ES cell lines that retain the potential to form chimaeric mice. Expand
Embryo-derived stem cells: of mice and men.
  • A. Smith
  • Biology, Medicine
  • Annual review of cell and developmental biology
  • 2001
The relationships between mouse embryonic stem cells, resident pluripotent cells in the embryo, and human embryo-derived cell lines are evaluated, and the prospects and challenges of embryo stem cell research are considered. Expand
Blastocyst implantation depends on maternal expression of leukaemia inhibitory factor
It is reported that transient expression of LIF in mice is essential for implantation, and females lacking a functional LIF gene are fertile, but their blastocysts fail to implant and do not develop. Expand