Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins

Abstract

Targeted protein degradation is a powerful tool in determining the function of specific proteins or protein complexes. We fused nanobodies to SPOP, an adaptor protein of the Cullin-RING E3 ubiquitin ligase complex, resulting in rapid ubiquitination and subsequent proteasome-dependent degradation of specific nuclear proteins in mammalian cells and zebrafish… (More)
DOI: 10.1038/srep14269

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