Nanobodies to Study G Protein-Coupled Receptor Structure and Function.

  title={Nanobodies to Study G Protein-Coupled Receptor Structure and Function.},
  author={Aashish Manglik and Brian K. Kobilka and Jan Steyaert},
  journal={Annual review of pharmacology and toxicology},
Ligand-induced activation of G protein-coupled receptors (GPCRs) is a key mechanism permitting communication between cells and organs. Enormous progress has recently elucidated the structural and dynamic features of GPCR transmembrane signaling. Nanobodies, the recombinant antigen-binding fragments of camelid heavy-chain-only antibodies, have emerged as important research tools to lock GPCRs in particular conformational states. Active-state stabilizing nanobodies have elucidated several agonist… 

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