NanoRNAs prime transcription initiation in vivo.

@article{Goldman2011NanoRNAsPT,
  title={NanoRNAs prime transcription initiation in vivo.},
  author={Seth R. Goldman and Josh S Sharp and Irina O. Vvedenskaya and Jonathan Livny and Simon L. B. Dove and Bryce E Nickels},
  journal={Molecular cell},
  year={2011},
  volume={42 6},
  pages={
          817-25
        }
}
It is often presumed that, in vivo, the initiation of RNA synthesis by DNA-dependent RNA polymerases occurs using NTPs alone. Here, using the model Gram-negative bacterium Pseudomonas aeruginosa, we demonstrate that depletion of the small-RNA-specific exonuclease, Oligoribonuclease, causes the accumulation of oligoribonucleotides 2 to ∼4 nt in length, "nanoRNAs," which serve as primers for transcription initiation at a significant fraction of promoters. Widespread use of nanoRNAs to prime… CONTINUE READING

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