NanoLC/ESI+ HRMS3 quantitation of DNA adducts induced by 1,3-butadiene.

  title={NanoLC/ESI+ HRMS3 quantitation of DNA adducts induced by 1,3-butadiene.},
  author={Dewakar Sangaraju and Peter W Villalta and Susith Wickramaratne and James W Swenberg and Natalia Y Tretyakova},
  journal={Journal of the American Society for Mass Spectrometry},
  volume={25 7},
Human exposure to 1,3-butadiene (BD) present in automobile exhaust, cigarette smoke, and forest fires is of great concern because of its potent carcinogenicity. The adverse health effects of BD are mediated by its epoxide metabolites such as 3,4-epoxy-1-butene (EB), which covalently modify genomic DNA to form promutagenic nucleobase adducts. Because of their direct role in cancer, BD-DNA adducts can be used as mechanism-based biomarkers of BD exposure. In the present work, a mass spectrometry… CONTINUE READING