Nano-magnesium aided activity enhancement and biophysical characterization of a psychrophilic α-amylase immobilized on graphene oxide nanosupport.

@article{Dutta2017NanomagnesiumAA,
  title={Nano-magnesium aided activity enhancement and biophysical characterization of a psychrophilic α-amylase immobilized on graphene oxide nanosupport.},
  author={Nalok Dutta and Subrata Kumar Biswas and Malay Kumar Saha},
  journal={Journal of bioscience and bioengineering},
  year={2017},
  volume={124 1},
  pages={15-22}
}
In the current literature we have devised an immobilization technique for conferring psychrostability to a cold active α-amylase (amy) enzyme by the use of magnesium nanoparticle (MgNP) and graphene oxide (GO). The GO-MgNP-amy nanocomposite showed enhanced enzymatic activity and thermostability at both upper (90°C) and lower (8°C) temperature extremes. The GO-MgNP-amy showed increased affinity towards substrate, reflected in the decrease in its Km by 2.35 and 14.9-fold at 8°C and 90°C… CONTINUE READING

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