Na(+)-H+ exchanger in isolated epithelial tracheal cells from sheep. Involvement in tracheal proton secretion.


The presence of a Na(+)-H+ exchanger in isolated tracheal epithelial cells has been tested. Sodium uptake in tracheocytes was found to increase when the intracellular medium was made acidic. Micromolar 5-(N-ethyl-N-isopropyl) amiloride (EIPA) concentrations reduced the sodium uptake in acidified cells. Intracellular pH was studied using the fluorescent… (More)