Na+-independent, electrogenic Cl- uptake across the posterior gills of the Chinese crab (Eriocheir sinensis): Voltage-clamp and microelectrode studies

@article{Onken2004NaindependentEC,
  title={Na+-independent, electrogenic Cl- uptake across the posterior gills of the Chinese crab (Eriocheir sinensis): Voltage-clamp and microelectrode studies},
  author={Horst Onken and Kai Graszynski and Wolfgang Zeiske},
  journal={Journal of Comparative Physiology B},
  year={2004},
  volume={161},
  pages={293-301}
}
Single gill lamellae from posterior gills of Chinese crabs (Eriocheir sinensis) were isolated, separated into halves and mounted in a modified Ussing chamber. Area-related short-circuit current (Isc) and conductance (Gtot) of this preparation were measured. Epithelial cells were impaled with microelectrodes through the basolateral membrane and cellular potentials (Vi under open- and Vsc under short-circuit conditions) as well as the voltage divider ratios (Fi, Fo) were determined. With NaCl… CONTINUE READING
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