Na+/H+ exchanger‐dependent intracellular alkalinization is an early event in malignant transformation and plays an essential role in the development of subsequent transformation‐associated phenotypes

@article{Reshkin2000NaHEI,
  title={Na+/H+ exchanger‐dependent intracellular alkalinization is an early event in malignant transformation and plays an essential role in the development of subsequent transformation‐associated phenotypes},
  author={Stephan Joel Reshkin and Antonia Bellizzi and Sandra Caldeira and Valentina Albarani and Ilaria Malanchi and Manuela Poignee and Marianna Alunni-Fabbroni and Valeria Casavola and Massimo Tommasino},
  journal={The FASEB Journal},
  year={2000},
  volume={14},
  pages={2185 - 2197}
}
In this study we investigate the mechanism of intracellular pH change and its role in malignant transformation using the E7 oncogene of human papillomavirus type 16 (HPV16). Infecting NIH3T3 cells with recombinant retroviruses expressing the HPV16 E7 or a transformation deficient mutant we show that alkalinization is transformation specific. In NIH3T3 cells in which transformation can be turned on and followed by induction of the HPV16 E7 oncogene expression, we demonstrate that cytoplasmic… 

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