Na+/Ca2+ exchange in rat osteoblast-like UMR 106 cells.

  title={Na+/Ca2+ exchange in rat osteoblast-like UMR 106 cells.},
  author={Kenneth E. White and Frank A. Gesek and Peter A Friedman},
  journal={Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research},
  volume={11 11},
Ca2+ efflux from osteoblasts is thought to be mediated by Na+/Ca2+ exchange and by a plasma membrane Ca(2+)-ATPase. The presence of plasma membrane Na+/Ca2+ exchange was determined in rat UMR 106 osteosarcoma cells by functional and molecular studies. Na+/Ca2+ exchange activity was tested by measuring changes of [Ca2+]i in single cells. After Na+ loading the cells and removing extracellular Na+, the direction of exchange was reversed and [Ca2+]i increased by 100%. Multiple isoforms of the NCX1… CONTINUE READING