NSs Encoded by Groundnut Bud Necrosis Virus Is a Bifunctional Enzyme

@inproceedings{Lokesh2010NSsEB,
  title={NSs Encoded by Groundnut Bud Necrosis Virus Is a Bifunctional Enzyme},
  author={Bhushan Lokesh and Panigrahi R. Rashmi and Bhat S. Amruta and Dharmaiah Srisathiyanarayanan and Mathur Ramabhadrashastry Narasimha Murthy and Handanahal Subbarao Savithri},
  booktitle={PloS one},
  year={2010}
}
Groundnut bud necrosis virus (GBNV), a member of genus Tospovirus in the family Bunyaviridae, infects a large number of leguminosae and solanaceae plants in India. With a view to elucidate the function of nonstructural protein, NSs encoded by the small RNA genome (S RNA), the NSs protein of GBNV- tomato (Karnataka) was over-expressed in E. coli and purified by Ni-NTA chromatography. The purified rNSs protein exhibited an RNA stimulated NTPase activity. Further, this activity was metal ion… CONTINUE READING