NMR study of the reconstitution of the beta-sheet of thioredoxin by fragment complementation.

@article{Tasayco1995NMRSO,
  title={NMR study of the reconstitution of the beta-sheet of thioredoxin by fragment complementation.},
  author={M. L. Tasayco and K Chao},
  journal={Proteins},
  year={1995},
  volume={22 1},
  pages={41-4}
}
The study of complementary protein fragments is thought to be generally useful to identify early folding intermediates. A prerequisite for these studies is the reconstitution of the native-like structure by fragment complementation. Structural analysis of the complementation of the domain-sized proteolytic fragments of E. coli thioredoxin, using a combination of H-exchange and 2D NMR experiments as a fingerprint technique, provide evidence for the extensive reconstitution of a native beta-sheet… CONTINUE READING