NMR study of the preferred membrane orientation of polyisoprenols (dolichol) and the impact of their complex with polyisoprenyl recognition sequence peptides on membrane structure.

@article{Zhou2005NMRSO,
  title={NMR study of the preferred membrane orientation of polyisoprenols (dolichol) and the impact of their complex with polyisoprenyl recognition sequence peptides on membrane structure.},
  author={Guo-Ping Zhou and F. A. Troy},
  journal={Glycobiology},
  year={2005},
  volume={15 4},
  pages={
          347-59
        }
}
Earlier NMR studies showed that the polyisoprenols (PIs) dolichol (C95), dolichylphosphate (C95-P) and undecaprenylphosphate (C55-P) could alter membrane structure by inducing in the lamellar phospholipid (PL) bilayer a nonlamellar or hexagonal (Hex II) structure. The destabilizing effect of C95 and C95-P on host fatty acyl chains was supported by small angle X-ray diffraction and freeze-fracture electron microscopy. Our present 1H- and 31P-NMR studies show that the addition of a polyisoprenol… Expand
The effect of dolichol on the structure and phase behaviour of phospholipid model membranes
TLDR
The data suggest that effect of dolichol on the phospholipid model membranes depend on both the head group and the hydrocarbon chains of the phosphoipid molecules, which is useful to understand the function of dlichol in cell membranes. Expand
Lipid-linked oligosaccharides in membranes sample conformations that facilitate binding to oligosaccharyltransferase.
TLDR
Molecular docking of the bacterial LLO to a bacterial OST suggests that such orientations can enhance binding of LLOs to OST. Expand
Configuration of polyisoprenoids affects the permeability and thermotropic properties of phospholipid/polyisoprenoid model membranes.
TLDR
Results of these experiments and the concomitant occurrence of alloprenols and ficaprenols in plant tissues suggest that cis/trans isomerization of the α-residue of polyisoprenoid molecule might comprise a putative mechanism responsible for modulation of the permeability of cellular membranes. Expand
Poly-Saturated Dolichols from Filamentous Fungi Modulate Activity of Dolichol-Dependent Glycosyltransferase and Physical Properties of Membranes
TLDR
It is confirmed using an LC-ESI-QTOF-MS analysis, that poly-saturated dolichols are abundant in other filamentous fungi, Trichoderma reesei, A. nidulans and Neurospora crassa, while the yeast Saccharomyces cerevisiae only contains the typical mono-s saturated dlichols. Expand
Structural studies and mechanism of Saccharomyces cerevisiae dolichyl-phosphate-mannose synthase: insights into the initial step of synthesis of dolichyl-phosphate-linked oligosaccharide chains in membranes of endoplasmic reticulum.
TLDR
The FRET analysis was used to characterize the functional properties of the enzyme and to evaluate its modeled structure, allowing for proposing a molecular mechanism of catalysis as an inverting mechanism of mannosyl residue transfer. Expand
Investigation of the conserved reentrant membrane helix in the monotopic phosphoglycosyl transferase superfamily supports key molecular interactions with polyprenol phosphate substrates.
TLDR
A multipronged biochemical and biophysical study of PglC, a monotopic phosphoglycosyl transferase that catalyzes the first membrane-committed step in N-linked glycoprotein biosynthesis in Campylobacter jejuni, and probes the significance of polyprenol phosphate geometry both in mediating substrate binding to PGLC and in modulating the local membrane environment. Expand
Lipid bilayer nanodisc platform for investigating polyprenol-dependent enzyme interactions and activities
TLDR
This work presents the functional reconstitution of two enzymes responsible for the early membrane-committed steps in glycan assembly by investigating initial steps from the Campylobacter jejuni N-linked glycosylation pathway, and provides a proof-of-concept demonstrating that nanodiscs can be used for the precise manipulation and study of polyprenol-dependent pathways. Expand
Substrate Specificity and Membrane Topology of Escherichia coli PgpB, an Undecaprenyl Pyrophosphate Phosphatase*
TLDR
It is hypothesize that the phospholipids are important determinants to ensure proper conformation of the atypical long axis C55 carrier lipid in membranes and that the in vitro C55-PP phosphatase activity of PgpB was specifically enhanced by different phospholIPids. Expand
Undecaprenyl Phosphate Synthesis.
TLDR
It was alsorecently shown that LpxTcatalyzes the specific transfer of the phosphate group arising from C55-PP to the lipidA moiety of lipopolysaccharides, leading to a lipid-A 1-diphosphate form which accounts for one-third of the total lipidA in wild-type E. coli cells. Expand
Structural basis for dolichylphosphate mannose biosynthesis
TLDR
Three high-resolution crystal structures of archaeal DPMS from Pyrococcus furiosus are reported, in complex with nucleotide, donor, and glycolipid product to provide insight into the mechanism of Dol-P-Man synthesis. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 63 REFERENCES
Nuclear magnetic resonance studies of polyisoprenols in model membranes.
2H-NMR investigation of polyisoprenols (PIs) in model membranes has revealed information about their motions, relative order, and locale within the membrane. Initial 2H-NMR studies of theExpand
2H NMR investigation of the organization and dynamics of polyisoprenols in membranes.
TLDR
Results of deuterium NMR investigations of site specifically 2H-labeled PIs incorporated into model membranes suggest that both ends of the esterified PI molecules see similar environments, probably in the bilayer interior, and suggest that the Esterified PIs studied here do not appear to adopt a conventional head group-at-interface orientation of lipids within the bilayers. Expand
Characterization by NMR and molecular modeling of the binding of polyisoprenols and polyisoprenyl recognition sequence peptides: 3D structure of the complexes reveals sites of specific interactions.
TLDR
These studies represent the first evidence for a direct physical interaction between specific contact amino acids in the PIRS peptides and the PI and supports the hypothesis of a bifunctional role for the PIs. Expand
Chemical synthesis and 2H NMR investigations of polyisoprenols: dynamics in model membranes.
TLDR
A synthetic route used for obtaining specifically labeled [omega,omega-(C2H3)2]PIs that initiates with the selective oxidation of the omega-terminal double bond of the PI with N-bromosuccinimide is presented. Expand
The influence of dolichol, dolichol esters, and dolichyl phosphate on phospholipid polymorphism and fluidity in model membranes.
TLDR
These studies suggest that dolichol and dlichol derivatives destabilize unsaturated phosphatidylethanolamine containing bilayer structures and promote hexagonal II phase formation. Expand
Paramagnetic isoprenoid carrier lipids. 1. Chemical synthesis and incorporation into model membranes.
TLDR
Electron spin resonance spectra of the phosphodiesters are matrix dependent, indicating rapid isotropic tumbling in chloroform but highly anisotropic reorientation in unsaturated phosphatidylcholine (PC) hosts. Expand
The conformation of dolichol.
TLDR
Study of the conformations of apparent least energy of dolichol-19 and smaller polymers of isoprene and the small-angle X-ray scattering (SAXS) method is employed to determine the validity of the apparent leastEnergy conformer, providing the first quantitative measure of the effect of dlichol on solution fluidity of a membrane-mimetic solvent. Expand
Interaction of cytochrome c with mixed dimyristoylphosphatidylcholine-dimyristoylphosphatidylserine bilayers: a deuterium nuclear magnetic resonance study.
TLDR
Partially relaxed spectroscopy allowed us to assign this slow component of protein addition to the methyl group and C2H2 groups near the methyl end of the chains, i.e., far from the binding sites of the extrinsic protein. Expand
Hamster UDP-N-Acetylglucosamine:Dolichol-P N-Acetylglucosamine-1-P Transferase Has Multiple Transmembrane Spans and a Critical Cytosolic Loop*
TLDR
Results directly support the hypothesis that dolichol-P- P-oligosaccharide assembly is initiated in the cytosol and that a downstream intermediate must translocate to the lumenal face of the ER membrane. Expand
Paramagnetic isoprenoid carrier lipids. 2. Dispersion and dynamics in lipid membranes.
TLDR
Transposition rates of phosphorylated species in small (300 A) or large (greater than or equal to 1000 A) diameter unilamellar phosphatidylcholine (PC) vesicles are slow, arguing against their proposed role in the transbilayer portage of sugar units during polysaccharide and glycoprotein assembly. Expand
...
1
2
3
4
5
...