NMR studies of the active site of isopenicillin N synthase, a non-heme iron(II) enzyme.

@article{Ming1991NMRSO,
  title={NMR studies of the active site of isopenicillin N synthase, a non-heme iron(II) enzyme.},
  author={L J Ming and Lawrence Que and Aidas V Kriauciunas and Charles A. Frolik and Victor J. Chen},
  journal={Biochemistry},
  year={1991},
  volume={30 50},
  pages={11653-9}
}
The active site structure of isopenicillin N synthase (IPNS) has been previously studied by the use of Mössbauer, EPR, electronic absorption, and NMR spectroscopies [Chen, V.J., Frolik, C.A., Orville, A.M., Harpel, M.R., Lipscomb, J.D., Surerus, K.K., & Münck, E. (1989) J. Biol. Chem. 264, 21677-21681; Ming, L.-J., Que, L., Jr., Kriauciunas, A., Frolik, C.A., & Chen, V.J. (1990) Inorg. Chem. 26, 1111-1112]. These studies have revealed three coordinated His residues along with three sites for… CONTINUE READING