NMR studies of cooperativity in the tetrahaem cytochrome c3 from Desulfovibrio vulgaris.

@article{Turner1996NMRSO,
  title={NMR studies of cooperativity in the tetrahaem cytochrome c3 from Desulfovibrio vulgaris.},
  author={David L. Turner and Carlos A Salgueiro and Teresa Catarino and Jean Legall and Ant{\'o}nio V. Xavier},
  journal={European journal of biochemistry},
  year={1996},
  volume={241 3},
  pages={723-31}
}
The thermodynamic properties of the Desulfovibrio vulgaris (Hildenborough) tetrahaem cytochrome c3 (Dvc3) are rationalised by a model which involves both homotropic (e-/e-) and heterotropic (e-/H+) cooperativity. The paramagnetic shifts of a methyl group from each haem of the Dvc3 have been determined in each stage of oxidation at several pH values by means of two-dimensional exchange NMR. The thermodynamic parameters are obtained by fitting the model to the NMR data and to redox titrations… CONTINUE READING