NMR studies of a bacterial cell culture medium (LB broth): cyclic nucleotides in yeast extracts.

@article{Rayner1990NMRSO,
  title={NMR studies of a bacterial cell culture medium (LB broth): cyclic nucleotides in yeast extracts.},
  author={Michael H. Rayner and Peter J Sadler and Michael D. Scawen},
  journal={FEMS microbiology letters},
  year={1990},
  volume={56 1-2},
  pages={217-21}
}
The composition of LB broth (tryptone, yeast extract and NaCl) was investigated by 1H,31P-NMR spectroscopy, FPLC and gel electrophoresis. An unexpected finding was the high level of 2'3'-cyclic nucleotides, detected by characteristic 31P-NMR resonances in the region 20-21 ppm, originating from the yeast component. 31P-NMR resonances for cyclic nucleotides were observed during the autolysis of Saccharomyces cerevisiae cells, and in model reactions of RNase with RNA.