NMR studies of a DNA containing 8-hydroxydeoxyguanosine.

  title={NMR studies of a DNA containing 8-hydroxydeoxyguanosine.},
  author={Yasuo Oda and S. Uesugi and Morio Ikehara and Susumu Nishimura and Yasutoshi Kawase and Hiroshi Ishikawa and Hideo Inoue and Eiko Ohtsuka},
  journal={Nucleic acids research},
  volume={19 7},
The effects of hydroxylation at the C8 of a deoxyguanosine residue in DNA were studied by NMR analysis of a self-complementary dodecanucleotide, d(C1-G2-C3-oh8G4-A5-A6-T7-T8-C9-G10-C11-G12), which has an 8-hydroxy-2'-deoxyguanosine (oh8dG) residue at the 4th position. NMR data indicate that the 8-hydroxyguanine (oh8G) base takes a 6,8-diketo tautomeric form and is base-paired to C with Watson-Crick type hydrogen bonds in a B-form structure. The thermal stability of the duplex is reduced, but… CONTINUE READING