NMR methods for in situ biofilm metabolism studies.

@article{Majors2005NMRMF,
  title={NMR methods for in situ biofilm metabolism studies.},
  author={Paul D. Majors and Jeffrey S. McLean and Grigoriy E. Pinchuk and James K. Fredrickson and Yuri Alan Gorby and Kevin R. Minard and Robert A. Wind},
  journal={Journal of microbiological methods},
  year={2005},
  volume={62 3},
  pages={
          337-44
        }
}
Novel procedures and instrumentation are described for nuclear magnetic resonance (NMR) spectroscopy and imaging studies of live, in situ microbial films. A perfused NMR/optical microscope sample chamber containing a planar biofilm support was integrated into a recirculation/dilution flow loop growth reactor system and used to grow in situ Shewanella oneidensis strain MR-1 biofilms. Localized NMR techniques were developed and used to non-invasively monitor time-resolved metabolite… CONTINUE READING
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