NMR and photo-CIDNP studies of human proinsulin and prohormone processing intermediates with application to endopeptidase recognition.

@article{Weiss1990NMRAP,
  title={NMR and photo-CIDNP studies of human proinsulin and prohormone processing intermediates with application to endopeptidase recognition.},
  author={Michael A Weiss and Bruce H. Frank and Igor Khait and Allen H. Pekar and R E Heiney and Steven E. Shoelson and L J Neuringer},
  journal={Biochemistry},
  year={1990},
  volume={29 36},
  pages={8389-401}
}
The proinsulin-insulin system provides a general model for the proteolytic processing of polypeptide hormones. Two proinsulin-specific endopeptidases have been defined, a type I activity that cleaves the B-chain/C-peptide junction (Arg31-Arg32) and a type II activity that cleaves the C-peptide/A-chain junction (Lys64-Arg65). These endopeptidases are specific for their respective dibasic target sites; not all such dibasic sites are cleaved, however, and studies of mutant proinsulins have… CONTINUE READING