NMR analysis of a 900K GroEL–GroES complex

  title={NMR analysis of a 900K GroEL–GroES complex},
  author={Jocelyne Fiaux and Eric B. Bertelsen and Arthur L. Horwich and Kurt W{\"u}thrich},
Biomacromolecular structures with a relative molecular mass (Mr) of 50,000 to 100,000 (50K–100K) have been generally considered to be inaccessible to analysis by solution NMR spectroscopy. Here we report spectra recorded from bacterial chaperonin complexes ten times this size limit (up to Mr 900K) using the techniques of transverse relaxation-optimized spectroscopy and cross-correlated relaxation-enhanced polarization transfer. These techniques prevent deterioration of the NMR spectra by the… 

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