NMN adenylyltransferase from bull testis: purification and properties.

@article{Balducci1995NMNAF,
  title={NMN adenylyltransferase from bull testis: purification and properties.},
  author={Enrico Balducci and Giuseppe Orsomando and Valeria Polzonetti and Alberto Vita and Monica Emanuelli and Nadia Raffaelli and Silverio Ruggieri and Giulio Magni and Paolo Natalini},
  journal={The Biochemical journal},
  year={1995},
  volume={310 ( Pt 2)},
  pages={
          395-400
        }
}
  • Enrico Balducci, Giuseppe Orsomando, +6 authors Paolo Natalini
  • Published in The Biochemical journal 1995
  • Chemistry, Medicine
  • The purification procedure of NMN adenylyltransferase from bull testis presented here consists of a heat step and an acidic precipitation followed by four chromatographic steps, including dye ligand, adsorption and hydrophobic chromatography. The final enzyme preparation subjected to non-denaturing and denaturating PAGE with silver nitrate staining exhibited a single band. At this step the enzyme appeared to be homogeneous. The M(r) value of the native enzyme calculated by gel filtration was… CONTINUE READING

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