NKBOR, a mini-Tn10-based transposon for random insertion in the chromosome of Gram-negative bacteria and the rapid recovery of sequences flanking the insertion sites in Escherichia coli.

@article{Rossignol2001NKBORAM,
  title={NKBOR, a mini-Tn10-based transposon for random insertion in the chromosome of Gram-negative bacteria and the rapid recovery of sequences flanking the insertion sites in Escherichia coli.},
  author={Mich{\`e}le Rossignol and Alan Basset and Olivier Esp{\'e}li and Fr{\'e}d{\'e}ric Boccard},
  journal={Research in microbiology},
  year={2001},
  volume={152 5},
  pages={
          481-5
        }
}
We have constructed an R6K-based suicide vector that permits the random insertion of a mini-transposon named NKBOR into the chromosome of Gram-negative bacteria and the subsequent rapid cloning of sequences flanking the insertion site in Escherichia coli. This mini-transposon contains a conditional R6K plasmid origin of replication, a kanamycin resistance gene and unique restriction sites between the IS10 inverted repeats. NKBOR can be propagated by replication in an E. coli strain containing… CONTINUE READING
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