NH3 permeability of principal cells and intercalated cells measured by confocal fluorescence imaging.

@article{Yip1995NH3PO,
  title={NH3 permeability of principal cells and intercalated cells measured by confocal fluorescence imaging.},
  author={K P Yip and Ira Kurtz},
  journal={The American journal of physiology},
  year={1995},
  volume={269 4 Pt 2},
  pages={F545-50}
}
The cortical collecting duct (CCD) is an important site for NH3 secretion in mammalian nephron. However, given the cellular heterogeneity of this epithelium, the transcellular sites for NH3 secretion are unknown. In the present study, a dual-excitation confocal microscope was designed and optimized to have sufficient temporal resolution to measure the permeability of ammonia (PNH3) across the basolateral and apical membrane of principal cells (PCs) and intercalated cells (ICs) in perfused… CONTINUE READING

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