NGL-2 Regulates Input-Specific Synapse Development in CA1 Pyramidal Neurons


An important organizing feature of the CNS is that individual neurons receive input from many different sources. Independent regulation of synaptic input is critical for the function and adaptive responses of the nervous system, but the underlying molecular mechanisms are not well understood. We identify the leucine-rich repeat (LRR)-containing protein NGL-2 (Lrrc4) as a key regulator of input-specific synapse development in the hippocampus. Using genetic deletion and shRNA-mediated knockdown, we demonstrate a role for NGL-2 in regulating the strength of synaptic transmission and spine density specifically at Schaffer collateral synapses in the stratum radiatum (SR) in CA1. NGL-2 protein is restricted to SR and spine regulation requires NGL-2's LRR and PDZ-binding domains. Finally, loss of NGL-2 disrupts cooperative interactions between distal and proximal synapses in CA1 pyramidal cells. These results demonstrate that NGL-2 is critical for pathway-specific synapse development and functional integration of distinct inputs.

DOI: 10.1016/j.neuron.2012.10.013

Extracted Key Phrases

8 Figures and Tables

Citations per Year

206 Citations

Semantic Scholar estimates that this publication has 206 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{DeNardo2012NGL2RI, title={NGL-2 Regulates Input-Specific Synapse Development in CA1 Pyramidal Neurons}, author={Laura A. DeNardo and Joris de Wit and Stefanie Otto-Hitt and Anirvan Ghosh}, journal={Neuron}, year={2012}, volume={76}, pages={762-775} }