NFATc3 regulates BK channel function in murine urinary bladder smooth muscle.

Abstract

The nuclear factor of activated T-cells (NFAT) is a Ca(2+)-dependent transcription factor that has been reported to regulate the expression of smooth muscle contractile proteins and ion channels. Here we report that large conductance Ca(2+)-sensitive potassium (BK) channels and voltage-gated K(+) (K(V)) channels may be regulatory targets of NFATc3 in urinary bladder smooth muscle (UBSM). UBSM myocytes from NFATc3-null mice displayed a reduction in iberiotoxin (IBTX)-sensitive BK currents, a decrease in mRNA for the pore-forming alpha-subunit of the BK channel, and a reduction in BK channel density compared with myocytes from wild-type mice. Tetraethylammonium chloride-sensitive K(V) currents were elevated in UBSM myocytes from NFATc3-null mice, as was mRNA for the Shab family member K(V)2.1. Despite K(V) current upregulation, bladder strips from NFATc3-null mice displayed an elevated contractile response to electrical field stimulation relative to strips from wild-type mice, but this difference was abrogated in the presence of the BK channel blocker IBTX. These results support a role for the transcription factor NFATc3 in regulating UBSM contractility, primarily through an NFATc3-dependent increase in BK channel activity.

DOI: 10.1152/ajpcell.00435.2007

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Cite this paper

@article{Layne2008NFATc3RB, title={NFATc3 regulates BK channel function in murine urinary bladder smooth muscle.}, author={Jeffrey J Layne and Michael Werner and David C. Hill-Eubanks and Mark T. Nelson}, journal={American journal of physiology. Cell physiology}, year={2008}, volume={295 3}, pages={C611-23} }