NF-E2 and GATA binding motifs are required for the formation of DNase I hypersensitive site 4 of the human beta-globin locus control region.

@article{Stamatoyannopoulos1995NFE2AG,
  title={NF-E2 and GATA binding motifs are required for the formation of DNase I hypersensitive site 4 of the human beta-globin locus control region.},
  author={John A. Stamatoyannopoulos and Aram Goodwin and Tobias Joyce and Christopher H. Lowrey},
  journal={The EMBO journal},
  year={1995},
  volume={14 1},
  pages={106-16}
}
The beta-like globin genes require the upstream locus control region (LCR) for proper expression. The active elements of the LCR coincide with strong erythroid-specific DNase I-hypersensitive sites (HSs). We have used 5' HS4 as a model to study the formation of these HSs. Previously, we identified a 101 bp element that is required for the formation of this HS. This element binds six proteins in vitro. We now report a mutational analysis of the HS4 HS-forming element (HSFE). This analysis… CONTINUE READING

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A combination of DNase I and micrococcal nuclease sensitivity assays indicates that the characteristic erythroid - specific hypersensitivity of HS4 to DNase I is the result of tissue - specific alterations in both nucleosome positioning and tertiary DNA structure .
A combination of DNase I and micrococcal nuclease sensitivity assays indicates that the characteristic erythroid - specific hypersensitivity of HS4 to DNase I is the result of tissue - specific alterations in both nucleosome positioning and tertiary DNA structure .
A combination of DNase I and micrococcal nuclease sensitivity assays indicates that the characteristic erythroid - specific hypersensitivity of HS4 to DNase I is the result of tissue - specific alterations in both nucleosome positioning and tertiary DNA structure .
A combination of DNase I and micrococcal nuclease sensitivity assays indicates that the characteristic erythroid - specific hypersensitivity of HS4 to DNase I is the result of tissue - specific alterations in both nucleosome positioning and tertiary DNA structure .
A combination of DNase I and micrococcal nuclease sensitivity assays indicates that the characteristic erythroid - specific hypersensitivity of HS4 to DNase I is the result of tissue - specific alterations in both nucleosome positioning and tertiary DNA structure .
A combination of DNase I and micrococcal nuclease sensitivity assays indicates that the characteristic erythroid - specific hypersensitivity of HS4 to DNase I is the result of tissue - specific alterations in both nucleosome positioning and tertiary DNA structure .
A combination of DNase I and micrococcal nuclease sensitivity assays indicates that the characteristic erythroid - specific hypersensitivity of HS4 to DNase I is the result of tissue - specific alterations in both nucleosome positioning and tertiary DNA structure .
A combination of DNase I and micrococcal nuclease sensitivity assays indicates that the characteristic erythroid - specific hypersensitivity of HS4 to DNase I is the result of tissue - specific alterations in both nucleosome positioning and tertiary DNA structure .
This analysis indicates that binding sites for the hematopoietic transcription factors NF - E2 and GATA-1 are required for the formation of the characteristic chromatin structure of the HS following stable transfection into murine erythroleukemia cells .
TRANSCRIPTION FACTORIs biochemical function of gene productNF-kappa B
This analysis indicates that binding sites for the hematopoietic transcription factors NF - E2 and GATA-1 are required for the formation of the characteristic chromatin structure of the HS following stable transfection into murine erythroleukemia cells .
This analysis indicates that binding sites for the hematopoietic transcription factors NF - E2 and GATA-1 are required for the formation of the characteristic chromatin structure of the HS following stable transfection into murine erythroleukemia cells .
This analysis indicates that binding sites for the hematopoietic transcription factors NF - E2 and GATA-1 are required for the formation of the characteristic chromatin structure of the HS following stable transfection into murine erythroleukemia cells .
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