NADPH‐diaphorase localization in the CNS and peripheral tissues of the predatory sea‐slug Pleurobranchaea californica

  title={NADPH‐diaphorase localization in the CNS and peripheral tissues of the predatory sea‐slug Pleurobranchaea californica},
  author={Leonid L. Moroz and Rhanor Gillette},
  journal={Journal of Comparative Neurology},
The distribution of putative nitric oxide synthase (NOS)‐containing cells in the opisthobranch mollusc Pleurobranchaea californica was studied histochemically via NADPH‐diaphorase (NADPH‐d) reduction of Nitro Blue Tetrazolium (NTB). Whole mounts and cryostat sections were prepared from the central nervous system and peripheral organs, including the buccal muscles, esophagus, salivary glands, foot, mantle, and gills. NADPH‐d‐positive neurons were localized predominantly to the buccal and pedal… 

Putative nitric oxide synthase containing nervous elements in male and female gonads of some marine bivalve mollusks revealed by NADPH-diaphorase histochemistry

NADPH-d histochemistry was used to reveal putative nitric oxide synthase (NOS)-containing nervous elements in the male and female gonads of Modiolus kurilensis, Crenomytilus grayanus, Anadara broughtoni, Mizuhopecten yessoensis, and Mercenaria stimpsoni, indicating a local regulatory apparatus formed by peripheral neurons and resembling a pattern of vegetative innervation in vertebrates.

Histochemical localization of NADPH-diaphorase-positive elements in the enteric nervous system of bivalve molluscs

Using a nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry, localization and morphology of putative nitric oxide synthase-containing elements were studied in the

Distribution of NADPH‐diaphorase reactivity and effects of nitric oxide on feeding and locomotory circuitry in the pteropod mollusc, Clione limacina

It is suggested that NO is an endogenous neuromodulator involved in the control of some aspects of feeding and locomotor behavior of Clione.


The bivalve species studied exhibit a similar distribution pattern of NADPH-d-positive cells, which lie separately or form small groups of two to three in the basal part of the epithelium, being most abundant in the dorsal and ventral regions.

Characterization of nitric oxidergic neurons in the alimentary tract of the snail Helix pomatia L.: Histochemical and physiological study

It is indicated that NO is an important signal molecule in the feeding system of Helix, involved, partially in cooperation with different molluscan neuropeptides, in the regulation of both neuronal and muscular activities.

Serotonin immunoreactivity in the central nervous system of the marine molluscs Pleurobranchaea californica and Tritonia diomedea

The clustering of 5‐HT‐IR neurons, their diverse axon pathways, and the known physiologic properties of their identified members are consistent with a loosely organized arousal system of serotonergic neurons whose components can be generally or differentially active in expression of diverse behaviors.

Serotonin‐immunoreactivity in peripheral tissues of the opisthobranch molluscs Pleurobranchaea californica and Tritonia diomedea

The distribution of serotonin (5‐HT)‐immunoreactive elements in peripheral organs of the sea‐slugs Pleurobranchaea californica and Tritonia diomedea was studied, suggesting a role for 5‐HT in sensory signaling.

Identifiable nitrergic neurons in the central nervous system of the nudibranch Melibe leonina localized with NADPH‐diaphorase histochemistry and nitric oxide synthase immunoreactivity

The results provide morphological evidence suggesting that NO may influence swimming or crawling in Melibe leonina and prove the role of pedal ganglia neurons in the control of locomotion.

The Stomatogastric and Enteric Nervous System of the Pulmonate Snail Megalobulimus abbreviatus: A Neurochemical Analysis

The GFAP immunoreaction in intramural plexuses suggests the presence of glial cells as an important component of ENS in this pulmonate snail.



Nitric Oxide Synthase Activity in the Molluscan CNS

The localization of relatively high NOS activity in neuron somata in the CNS of Pleurobranchaea is markedly different from the other opisthobranchs, all of which are grazers, potentially related to the animal's opportunistic predatory lifestyle.

Nitric oxide synthase-immunoreactive cells in the CNS and periphery of Lymnaea.

The presence and distribution of nitric oxide synthase in the CNS and peripheral organs of the pulmonate mollusc, Lymnaea stagnalis were studied using an antiserum developed against rat cerebellar NOS to support other recent reports indicating that NO may be a signal molecule in the brain of molluscs.

Is nitric oxide (NO) produced by invertebrate neurones?

Both histochemical and biochemical methods indicate that NO can be used as a signal molecule by specific neurones in advanced invertebrates.

Co-localization of NADPH-diaphorase and myomodulin in synaptic glomeruli of Aplysia.

The co-localization of myomodulin immunoreactivity and NADPH-diaphorase staining in the synaptic glomerulus, and the staining of select neurons and synaptic structures strongly suggests that nitric oxide functions in interneuronal communication.

Nitric oxide synthase and neuronal NADPH diaphorase are identical in brain and peripheral tissues.

The identity of neuronal NO synthase and NADPH diaphorase suggests a role for NO in modulating neurotoxicity, and is in line with previous work on neuronal messenger molecules.

Motor and sensory mechanisms of feeding in Pleurobranchaea.

A conception of the feeding cycle of proboscis extension, thrusting, and withdrawal, is developed by considering the muscular anatomy and by behavior simulation and was confirmed by recording motor nerve output and muscle activity during food stimulation.

Neuronal NADPH diaphorase is a nitric oxide synthase.

It is found that nitric oxide synthase activity and NAD PH diaphorase copurify to homogeneity and that both activities could be immunoprecipitated with an antibody recognizing neuronal NADPH diaphirase.

Calcium-activated release of nitric oxide and cellular distribution of nitric oxide-synthesizing neurons in the nervous system of the locust

  • U. MullerG. Bicker
  • Biology
    The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1994
The combined anatomical and biochemical experiments provide firm evidence that NO is a messenger molecule released in the CNS of the locust.

Neural control of swimming in Aplysia brasiliana. III. Serotonergic modulatory neurons.

A group of serotonergic neurons in the pedal ganglia of Aplysia brasiliana are described and their modulatory effects on the motoneuron input to swimming muscles of the parapodia are characterized and the possibility that EJP enhancement is presynaptic, postsynaptic, or a combination is left open.