NADP and NAD utilization in Haemophilus influenzae

@article{Reidl2000NADPAN,
  title={NADP and NAD utilization in Haemophilus influenzae},
  author={Joachim Reidl and Stefan Schlör and Anita Kraiss and J Schmidt-Brauns and G. Kemmer and E Soleva},
  journal={Molecular Microbiology},
  year={2000},
  volume={35}
}
Exogenous NAD utilization or pyridine nucleotide cycle metabolism is used by many bacteria to maintain NAD turnover and to limit energy‐dependent de novo NAD synthesis. The genus Haemophilus includes several important pathogenic bacterial species that require NAD as an essential growth factor. The molecular mechanisms of NAD uptake and processing are understood only in part for Haemophilus. In this report, we present data showing that the outer membrane lipoprotein e(P4), encoded by the hel… 

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References

SHOWING 1-10 OF 48 REFERENCES

Studies of NAD kinase and NMN:ATP adenylyltransferase in Haemophilus influenzae.

TLDR
The mononucleotides of 3-acetylpyridine and 3-aminopyridine were shown to be as effective as the corresponding din nucleotides in the support of growth and inhibition of growth of H. influenzae, respectively.

Characterization of Haemophilus influenzae nucleotide pyrophosphatase. An enzyme of critical importance for growth of the organism.

Lipoprotein e(P4) is essential for hemin uptake by Haemophilus influenzae

TLDR
A DNA fragment cloned from H. influenzae that allows an Escherichia coli hemA mutant to employ exogenous hemin or protoporphyrin IX as sole sources of porphyrIn is cloned and it is demonstrated that hel is essential for growth under aerobic conditions but not under anaerobic conditions.

Salmonella typhimurium mutants lacking NAD pyrophosphatase

TLDR
This work isolated and characterized mutants of S. typhimurium lacking NAD pyrophosphatase activity and identified mutants that carry mutations that map at a new locus, designated pnuE, between 86 and 87 min on the Salmonella chromosome.

Cytoplasmic Membrane Lipoprotein LppC ofStreptococcus equisimilis Functions as an Acid Phosphatase

  • H. Malke
  • Biology
    Applied and Environmental Microbiology
  • 1998
TLDR
A possible function for these proteins is suggested and a novel function of streptococcal cell membrane lipoproteins is established after significant structural homology of LppC to the Streptococcus pyogenes LppA, Flavobacterium meningosepticum OplA, Helicobacter pylori HP1285, and Haemophilus influenzae Hel [e (P4)] proteins.

Synthesis of nicotinamide mononucleotide by human erythrocytes in vitro.

Haemin and nicotinamide adenine dinucleotide requirements of Haemophilus influenzae and Haemophilus parainfluenzae.

TLDR
The two species were clearly differentiated by the requirement of H. influenzae strains for haemin on a proteose-peptone basal medium and with an inoculum of 1-20 cells, and the amount of NAD required was dissimilar.

Identification of the gene (aphA) encoding the class B acid phosphatase/phosphotransferase of Escherichia coli MG1655 and characterization of its product.

An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of

Codehydrogenase I and Other Pyridinium Compounds as V-Factor for Hemophilus influenzae and H. parainfluenzae.

TLDR
The findings indicate generally that the V-factor content of blood is not related to the intake of nicotinamide nor to deficiency thereof, but that it is reduced in liver and muscle of dogs suffering from blacktongue.