NADP and NAD utilization in Haemophilus influenzae

@article{Reidl2000NADPAN,
  title={NADP and NAD utilization in Haemophilus influenzae},
  author={Joachim Reidl and Stefan Schlör and Anita Kraiss and J Schmidt-Brauns and G. Kemmer and E Soleva},
  journal={Molecular Microbiology},
  year={2000},
  volume={35}
}
Exogenous NAD utilization or pyridine nucleotide cycle metabolism is used by many bacteria to maintain NAD turnover and to limit energy‐dependent de novo NAD synthesis. The genus Haemophilus includes several important pathogenic bacterial species that require NAD as an essential growth factor. The molecular mechanisms of NAD uptake and processing are understood only in part for Haemophilus. In this report, we present data showing that the outer membrane lipoprotein e(P4), encoded by the hel… 
Is a NAD pyrophosphatase activity necessary for Haemophilus influenzae type b multiplication in the blood stream?
TLDR
N of type b Eagan strains is involved in NAD uptake and in processing NAD to NR, which appears to be the substrate for an as yet unidentified cytoplasmic membrane NR transport system, and H. influenzae type b nadN mutants are able to survive in vivo in the five-day-old infant rat model of human invasive disease.
NadN and e (P4) Are Essential for Utilization of NAD and Nicotinamide Mononucleotide but Not Nicotinamide Riboside in Haemophilus influenzae
TLDR
The functions and substrates of these two proteins as they act together in an NAD utilization pathway are described, which indicate that NadN harbors not only NAD pyrophosphatase but also NMN 5'-nucleotidase activity.
Ribosylnicotinamide Kinase Domain of NadR Protein: Identification and Implications in NAD Biosynthesis
TLDR
A model for the complete salvage pathway from exogenous N -ribosylnicotinamide to NAD which involves the concerted action of the PnuC transporter and NRK, after is proposed.
Ribosylnicotinamide Kinase Domain of NadR Protein: Identification and Implications in NAD Biosynthesis
TLDR
A model for the complete salvage pathway from exogenous N-ribosylnicotinamide to NAD which involves the concerted action of the PnuC transporter and NRK, followed by the NMNAT activity of the NadR protein is proposed.
Coupling of NAD+ Biosynthesis and Nicotinamide Ribosyl Transport: Characterization of NadR Ribonucleotide Kinase Mutants of Haemophilus influenzae
TLDR
The data demonstrate that the NR phosphorylation step is essential for both NR uptake across the inner membrane and NAD+ synthesis and is also involved in controlling the NAD+ biosynthesis rate.
The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism.
TLDR
Sequence and structural analysis of H. influenzae NadN led us to discover that human CD73 is capable of processing both NAD and NMN, therefore disclosing a possible novel function of humanCD73 in systemic NAD metabolism.
Determining the Extremes of the Cellular NAD(H) Level by Using an Escherichia coli NAD+-Auxotrophic Mutant
TLDR
A strategy to determine the extreme NAD(H) levels in Escherichia coli cells that were genetically engineered to be NAD+ auxotrophic by disrupting the essential gene nadE, which is responsible for the last step of NAD+ biosynthesis in cells harboring the ntt4 gene is developed.
Porin OmpP2 of Haemophilus influenzae Shows Specificity for Nicotinamide-derived Nucleotide Substrates*
TLDR
OmpP2 was formerly described as a general diffusion porin, an additional property of acting as a facilitator for nicotinamide-based nucleotide transport may have evolved to support and optimize utilization of the essential cofactor sources NAD and NMN in H. influenzae.
The power to reduce: pyridine nucleotides--small molecules with a multitude of functions.
TLDR
Recent advances in the understanding of the biosynthesis and signalling functions of NAD(P) are summarized and new insights into the molecular mechanisms of NADPH generation and their roles in cell physiology are highlighted.
MJ0917 in Archaeon Methanococcus jannaschii Is a Novel NADP Phosphatase/NAD Kinase*
NAD kinase phosphorylates NAD+ to form NADP+. Conversely, NADP phosphatase, which has not yet been identified, dephosphorylates NADP+ to produce NAD+. Among the NAD kinase homologs, the primary
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References

SHOWING 1-10 OF 48 REFERENCES
Studies of NAD kinase and NMN:ATP adenylyltransferase in Haemophilus influenzae.
TLDR
The mononucleotides of 3-acetylpyridine and 3-aminopyridine were shown to be as effective as the corresponding din nucleotides in the support of growth and inhibition of growth of H. influenzae, respectively.
Characterization of Haemophilus influenzae nucleotide pyrophosphatase. An enzyme of critical importance for growth of the organism.
Lipoprotein e(P4) is essential for hemin uptake by Haemophilus influenzae
TLDR
A DNA fragment cloned from H. influenzae that allows an Escherichia coli hemA mutant to employ exogenous hemin or protoporphyrin IX as sole sources of porphyrIn is cloned and it is demonstrated that hel is essential for growth under aerobic conditions but not under anaerobic conditions.
Salmonella typhimurium mutants lacking NAD pyrophosphatase
TLDR
This work isolated and characterized mutants of S. typhimurium lacking NAD pyrophosphatase activity and identified mutants that carry mutations that map at a new locus, designated pnuE, between 86 and 87 min on the Salmonella chromosome.
Outer Membrane Lipoprotein e (P4) ofHaemophilus influenzae Is a Novel Phosphomonoesterase
TLDR
The identification, localization, purification, and characterization of a novel, surface-localized phosphomonoesterase from a nontypeable H. influenzae strain is described, suggesting a unique role for this unusual phosphohydrolase.
Cytoplasmic Membrane Lipoprotein LppC ofStreptococcus equisimilis Functions as an Acid Phosphatase
  • H. Malke
  • Biology
    Applied and Environmental Microbiology
  • 1998
TLDR
A possible function for these proteins is suggested and a novel function of streptococcal cell membrane lipoproteins is established after significant structural homology of LppC to the Streptococcus pyogenes LppA, Flavobacterium meningosepticum OplA, Helicobacter pylori HP1285, and Haemophilus influenzae Hel [e (P4)] proteins.
Haemin and nicotinamide adenine dinucleotide requirements of Haemophilus influenzae and Haemophilus parainfluenzae.
TLDR
The two species were clearly differentiated by the requirement of H. influenzae strains for haemin on a proteose-peptone basal medium and with an inoculum of 1-20 cells, and the amount of NAD required was dissimilar.
In vitro evaluation of nicotinamide riboside analogs against Haemophilus influenzae
TLDR
The antibacterial activity of 3-aminopyridine riboside was found to be specific for H. influenzae but had no effect on the growth of Staphylococcus aureus or Escherichia coli.
Identification of the gene (aphA) encoding the class B acid phosphatase/phosphotransferase of Escherichia coli MG1655 and characterization of its product.
An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of
...
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