NAD-binding site of the C3-like ADP-ribosyltransferase from Clostridium limosum.

  title={NAD-binding site of the C3-like ADP-ribosyltransferase from Clostridium limosum.},
  author={M C Jung and Ingo Just and Jozef Van Damme and Jo{\"e}l Vandekerckhove and Klaus Aktories},
  journal={The Journal of biological chemistry},
  volume={268 31},
Treatment of the Rho-ADP-ribosylating C3-like transferase from Clostridium limosum by ultraviolet irradiation in the presence of [carbonyl-14C]NAD incorporated 1 mol of label/mol of exoenzyme. Concomitantly, the transferase and NAD glycohydrolase activity was impaired. A peptide containing the radiolabel was obtained by proteolysis with either staphylococcal protease V8 or trypsin. Their amino acid sequences were Ala/Asp-Gly-Tyr-Ile-Glu-Pro-Ile-Ser-Thr-Phe-Lys-Gly-Gln-Leu-X-Val-Leu-Le u-Pro… CONTINUE READING


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