N-methyl-D-aspartate induces neurogranin/RC3 oxidation in rat brain slices.

  title={N-methyl-D-aspartate induces neurogranin/RC3 oxidation in rat brain slices.},
  author={Jie Jack Li and Jhang Ho Pak and Freesia L. Huang and K P Huang},
  journal={The Journal of biological chemistry},
  volume={274 3},
Neurogranin/RC3 (Ng), a postsynaptic neuronal protein kinase C (PKC) substrate, binds calmodulin (CaM) at low level of Ca2+. In vitro, rat brain Ng can be oxidized by nitric oxide (NO) donors and by oxidants to form an intramolecular disulfide bond with resulting downward mobility shift on nonreducing SDS-polyacrylamide gel electrophoresis. The oxidized Ng, as compared with the reduced form, is a poorer substrate of PKC but like the PKC-phosphorylated Ng has a lower affinity for CaM than the… CONTINUE READING