N-linked keratan sulfate in the aggrecan interglobular domain potentiates aggrecanase activity.

@article{Poon2005NlinkedKS,
  title={N-linked keratan sulfate in the aggrecan interglobular domain potentiates aggrecanase activity.},
  author={Christopher J Poon and Anna Plaas and Doug R Keene and David James McQuillan and Karena Last and Amanda J Fosang},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 25},
  pages={23615-21}
}
Keratan sulfate is thought to influence the cleavage of aggrecan by metalloenzymes. We have therefore produced a recombinant substrate, substituted with keratan sulfate, suitable for the study of aggrecanolysis in vitro. Recombinant human G1-G2 was produced in primary bovine keratocytes using a vaccinia virus expression system. Following purification and digestion with specific hydrolases, fluorophore-assisted carbohydrate electrophoresis was used to confirm the presence of the monosulfated Gal… CONTINUE READING
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