N-linked glycoprotein biosynthesis in the developing mouse embryo.

  title={N-linked glycoprotein biosynthesis in the developing mouse embryo.},
  author={David Randall Armant and Howard A. Kaplan and William J. Lennarz},
  journal={Developmental biology},
  volume={113 1},
We have developed microenzymic assays that have, for the first time, enabled analysis of several enzymes in the pathway for N-linked glycoprotein biosynthesis in pre- and peri-implantation mouse embryos. The in vitro activities of the glycosyl transferases responsible for the formation of N-acetylglucosaminylpyrophosphoryldolichol,N, N'-diacetyl-chitobiosylpyrophosphoryldolichol, mannosylphosphoryldolichol, and glucosylphosphoryldolichol were found to decrease after fertilization before… CONTINUE READING