N-glycosylation sites of plant purple acid phosphatases important for protein expression and secretion in insect cells.

@article{Olczak2007NglycosylationSO,
  title={N-glycosylation sites of plant purple acid phosphatases important for protein expression and secretion in insect cells.},
  author={Mariusz Olczak and T Olczak},
  journal={Archives of biochemistry and biophysics},
  year={2007},
  volume={461 2},
  pages={247-54}
}
Analysis of plant purple acid phosphatases (PAPs) showed high conservation and different distribution of N-glycosylation sites. Oligosaccharide structures of Lupinus luteus acid phosphatase (Lu_AP) produced in insect cells were determined. Mutant Lu_AP and Phaseolus vulgaris (Ph_AP) phosphatases lacking possibility of N-glycosylation at highly conserved sites were generated and expressed in insect cells. A role for N-glycosylation in the stability of PAPs was indicated by unsuccessful attempts… CONTINUE READING