N-cadherin-mediated human granulosa cell adhesion prevents apoptosis: a role in follicular atresia and luteolysis?

@article{Makrigiannakis1999NcadherinmediatedHG,
  title={N-cadherin-mediated human granulosa cell adhesion prevents apoptosis: a role in follicular atresia and luteolysis?},
  author={Antonis Makrigiannakis and George Coukos and Melpo Christofidou-Solomidou and Barbara J. Gour and Glenn L Radice and Orest W. Blaschuk and Christos Coutifaris},
  journal={The American journal of pathology},
  year={1999},
  volume={154 5},
  pages={1391-406}
}
Studies suggest that cell-cell interactions may regulate apoptosis, and in particular, the calcium-dependent cell adhesion molecule N-cadherin has been shown to be capable of modulating this process. Rat granulosa cells (GCs) are known to express N-cadherin whereas cAMP is known to induce apoptosis in human and rat GCs. Based on these observations, we hypothesized that N-cadherin regulates human GC apoptosis via a cAMP-dependent mechanism. N-cadherin expression was evaluated in ovarian… CONTINUE READING

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Studies suggest that cell - cell interactions may regulate apoptosis , and in particular , the calcium - dependent cell adhesion molecule N - cadherin has been shown to be capable of modulating this process .
Studies suggest that cell - cell interactions may regulate apoptosis , and in particular , the calcium - dependent cell adhesion molecule N - cadherin has been shown to be capable of modulating this process .
Studies suggest that cell - cell interactions may regulate apoptosis , and in particular , the calcium - dependent cell adhesion molecule N - cadherin has been shown to be capable of modulating this process .
Studies suggest that cell - cell interactions may regulate apoptosis , and in particular , the calcium - dependent cell adhesion molecule N - cadherin has been shown to be capable of modulating this process .
Apoptosis was assessed by TUNEL and DNA fragmentation analysis and confirmed by flow cytometric cell cycle analysis and electron microscopy .
Apoptosis was assessed by TUNEL and DNA fragmentation analysis and confirmed by flow cytometric cell cycle analysis and electron microscopy .
N - cadherin was weak in atretic follicles and regressing corpora lutea .
N - cadherin was weak in atretic follicles and regressing corpora lutea .
GCs in situ stained intensely for N - cadherin in preantral and normal growing preovulatory follicles as well as early corpora lutea .
N - cadherin expression was evaluated in ovarian follicles and corpora lutea utilizing immunohistochemical techniques and in luteinized GCs in culture using immunoblotting , flow cytometric analysis , immunohistochemistry , and indirect immunofluorescence techniques utilizing anti - N - cadherin antibodies directed against both the extracellular and cytoplasmic domains of the molecule .
N - cadherin expression was evaluated in ovarian follicles and corpora lutea utilizing immunohistochemical techniques and in luteinized GCs in culture using immunoblotting , flow cytometric analysis , immunohistochemistry , and indirect immunofluorescence techniques utilizing anti - N - cadherin antibodies directed against both the extracellular and cytoplasmic domains of the molecule .
N - cadherin expression was evaluated in ovarian follicles and corpora lutea utilizing immunohistochemical techniques and in luteinized GCs in culture using immunoblotting , flow cytometric analysis , immunohistochemistry , and indirect immunofluorescence techniques utilizing anti - N - cadherin antibodies directed against both the extracellular and cytoplasmic domains of the molecule .
N - cadherin was weak in atretic follicles and regressing corpora lutea .
GCs in situ stained intensely for N - cadherin in preantral and normal growing preovulatory follicles as well as early corpora lutea .
N - cadherin was weak in atretic follicles and regressing corpora lutea .
N - cadherin expression was evaluated in ovarian follicles and corpora lutea utilizing immunohistochemical techniques and in luteinized GCs in culture using immunoblotting , flow cytometric analysis , immunohistochemistry , and indirect immunofluorescence techniques utilizing anti - N - cadherin antibodies directed against both the extracellular and cytoplasmic domains of the molecule .
N - cadherin was weak in atretic follicles and regressing corpora lutea .
N - cadherin was weak in atretic follicles and regressing corpora lutea .
GCs in situ stained intensely for N - cadherin in preantral and normal growing preovulatory follicles as well as early corpora lutea .
N - cadherin expression was evaluated in ovarian follicles and corpora lutea utilizing immunohistochemical techniques and in luteinized GCs in culture using immunoblotting , flow cytometric analysis , immunohistochemistry , and indirect immunofluorescence techniques utilizing anti - N - cadherin antibodies directed against both the extracellular and cytoplasmic domains of the molecule .
N - cadherin expression was evaluated in ovarian follicles and corpora lutea utilizing immunohistochemical techniques and in luteinized GCs in culture using immunoblotting , flow cytometric analysis , immunohistochemistry , and indirect immunofluorescence techniques utilizing anti - N - cadherin antibodies directed against both the extracellular and cytoplasmic domains of the molecule .
N - cadherin expression was evaluated in ovarian follicles and corpora lutea utilizing immunohistochemical techniques and in luteinized GCs in culture using immunoblotting , flow cytometric analysis , immunohistochemistry , and indirect immunofluorescence techniques utilizing anti - N - cadherin antibodies directed against both the extracellular and cytoplasmic domains of the molecule .
N - cadherin was weak in atretic follicles and regressing corpora lutea .
GCs in situ stained intensely for N - cadherin in preantral and normal growing preovulatory follicles as well as early corpora lutea .
N - cadherin was weak in atretic follicles and regressing corpora lutea .
N - cadherin expression was evaluated in ovarian follicles and corpora lutea utilizing immunohistochemical techniques and in luteinized GCs in culture using immunoblotting , flow cytometric analysis , immunohistochemistry , and indirect immunofluorescence techniques utilizing anti - N - cadherin antibodies directed against both the extracellular and cytoplasmic domains of the molecule .
N - cadherin expression was evaluated in ovarian follicles and corpora lutea utilizing immunohistochemical techniques and in luteinized GCs in culture using immunoblotting , flow cytometric analysis , immunohistochemistry , and indirect immunofluorescence techniques utilizing anti - N - cadherin antibodies directed against both the extracellular and cytoplasmic domains of the molecule .
N - cadherin was weak in atretic follicles and regressing corpora lutea .
GCs in situ stained intensely for N - cadherin in preantral and normal growing preovulatory follicles as well as early corpora lutea .
N - cadherin was weak in atretic follicles and regressing corpora lutea .
N - cadherin expression was evaluated in ovarian follicles and corpora lutea utilizing immunohistochemical techniques and in luteinized GCs in culture using immunoblotting , flow cytometric analysis , immunohistochemistry , and indirect immunofluorescence techniques utilizing anti - N - cadherin antibodies directed against both the extracellular and cytoplasmic domains of the molecule .
Apoptosis was assessed by TUNEL and DNA fragmentation analysis and confirmed by flow cytometric cell cycle analysis and electron microscopy .
Apoptosis was assessed by TUNEL and DNA fragmentation analysis and confirmed by flow cytometric cell cycle analysis and electron microscopy .
N - cadherin was found to be expressed by aggregating GCs in vitro and GCs cultured in the presence of either N - cadherin function disrupting antibodies or peptides exhibiting enhanced rates of apoptosis .
N - cadherin expression was evaluated in ovarian follicles and corpora lutea utilizing immunohistochemical techniques and in luteinized GCs in culture using immunoblotting , flow cytometric analysis , immunohistochemistry , and indirect immunofluorescence techniques utilizing anti - N - cadherin antibodies directed against both the extracellular and cytoplasmic domains of the molecule .
N - cadherin expression was evaluated in ovarian follicles and corpora lutea utilizing immunohistochemical techniques and in luteinized GCs in culture using immunoblotting , flow cytometric analysis , immunohistochemistry , and indirect immunofluorescence techniques utilizing anti - N - cadherin antibodies directed against both the extracellular and cytoplasmic domains of the molecule .
N - cadherin was found to be expressed by aggregating GCs in vitro and GCs cultured in the presence of either N - cadherin function disrupting antibodies or peptides exhibiting enhanced rates of apoptosis .
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