N-acetyl-D-neuraminic acid synthesis in Escherichia coli K1 occurs through condensation of N-acetyl-D-mannosamine and pyruvate.

  title={N-acetyl-D-neuraminic acid synthesis in Escherichia coli K1 occurs through condensation of N-acetyl-D-mannosamine and pyruvate.},
  author={L. Rodr{\'i}guez-Aparicio and M. A. Ferrero and {\'A}. Reglero},
  journal={The Biochemical journal},
  volume={308 ( Pt 2)},
Two enzymes have been found to be involved in bacterial N-acetyl-D-neuraminic acid (NeuAc) synthesis: NeuAc synthase, which condenses N-acetyl-L,D-mannosamine and phosphoenolpyruvate, and NeuAc lyase or NeuAc aldolase, which condenses N-acetyl-D-mannosamine and pyruvate. When we used Escherichia coli K1 crude extracts, we observed the generation of NeuAc in the presence of N-acetylmannosamine and both phosphoenolpyruvate (NeuAc synthase activity) or pyruvate (NeuAc lyase activity). However… Expand
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This system provides a sensitive, rapid, reproducible, specific and simple procedure (feasible with commercial reagents) for measuring amino sugar 2'-epimerases from eukaryotic and prokaryotic sources. Expand
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The ManNAc and the GlcNAc phosphorylation that occurs during uptake affected NeuAc synthesis in vitro and account for the low in vivo PA production observed when E. coli K1 uses Man NAc or Glc NAc as a carbon source for growth. Expand